Paracetamol
and Salicylate
1.
Paracetamol (Acetaminophen) Assay
The
Paracetamol assay is an enzymatic assay
for the detection and quantitation of free
paracetamol in human sera or plasma.
Summary and explanation of test
Paracetamol
is a commonly used analgesic which, if taken
in excessive amounts, can lead to toxic
liver damage and, less commonly, renal impairment.
The
method is based on the use of an enzyme
specific for the amide bond of acylated
aromatic amines. It cleaves the paracetamol
molecule, yielding p-aminophenol, which
reacts specifically with o-cresol in ammonical
copper solution to produce a blue colour.
The assay is specific for the parent compound
and does not detect paracetamol metabolites.
Key
Points
-
SPECIFIC – gives no reaction with
the conjugated or common metabolites of
paracetamol.
-
RAPID
– assay may be performed manually
in 15mins.
-
ADAPTABLE
– kit can be run on discrete analysers.
-
EXCELLENT
RESULTS – performs consistently well
on independent quality assurance schemes.
-
ROBUST
– easy to use methodology.
The
various product formats are listed below:
Code |
Name |
Determinations |
IFU |
| K8001 |
Paracetamol Assay |
20 |
Download |
| K8002 |
Paracetamol Assay |
60 |
A list of all the protocols for clinical chemistry analysers is available at the bottom of this page. To request any of theses protocols, please contact us
2.
Salicylate Enzyme Assay
The
Salicylate assay is an enzymatic assay for
the detection and quantitation of salicylate
in human sera or plasma.
Salicylate
(aspirin) is a common non-steroidal drug used
for its analgesic and anti-inflammatory properties.
Ingestion of large amounts of salicylates
leads to disturbances of the central nervous
system and to gastrointestinal problems, encephalopathy
and renal failure. Salicylate intoxication
represents an acute medical emergency and
rapid diagnosis and quantitative determination
of the drug is necessary to assess effective
patient management.
The
assay utilises Salicylate Hydroxylase which
catalyses the conversion of salicylate and
NADH to catechol and NAD in the presence of
oxygen. The resulting decrease in absorbance
at 340nm, due to the conversion of NADH to
NAD, is directly proportional to the concentration
of Salicylate in the sample.
Salicylate
Hydroxylase
Salicylate + NADH + H+ ------->
Catechol + NAD+
‘Ready
to Use’ liquid reagents
