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Immunology Clinical Chemistry Urease Hp Tests Instrumentation

Paracetamol and Salicylate

1. Paracetamol (Acetaminophen) Assay

The Paracetamol assay is an enzymatic assay for the detection and quantitation of free paracetamol in human sera or plasma.

Summary and explanation of test

Paracetamol is a commonly used analgesic which, if taken in excessive amounts, can lead to toxic liver damage and, less commonly, renal impairment.

The method is based on the use of an enzyme specific for the amide bond of acylated aromatic amines. It cleaves the paracetamol molecule, yielding p-aminophenol, which reacts specifically with o-cresol in ammonical copper solution to produce a blue colour. The assay is specific for the parent compound and does not detect paracetamol metabolites.

Key Points

  • SPECIFIC – gives no reaction with the conjugated or common metabolites of paracetamol.
  • RAPID – assay may be performed manually in 15mins.
  • ADAPTABLE – kit can be run on discrete analysers.
  • EXCELLENT RESULTS – performs consistently well on independent quality assurance schemes.
  • ROBUST – easy to use methodology.

The various product formats are listed below:

Code
Name
Determinations
IFU
K8001 Paracetamol Assay
20

Download

K8002 Paracetamol Assay
60

A list of all the protocols for clinical chemistry analysers is available at the bottom of this page. To request any of theses protocols, please contact us

2. Salicylate Enzyme Assay

The Salicylate assay is an enzymatic assay for the detection and quantitation of salicylate in human sera or plasma.

Salicylate (aspirin) is a common non-steroidal drug used for its analgesic and anti-inflammatory properties. Ingestion of large amounts of salicylates leads to disturbances of the central nervous system and to gastrointestinal problems, encephalopathy and renal failure. Salicylate intoxication represents an acute medical emergency and rapid diagnosis and quantitative determination of the drug is necessary to assess effective patient management.

The assay utilises Salicylate Hydroxylase which catalyses the conversion of salicylate and NADH to catechol and NAD in the presence of oxygen. The resulting decrease in absorbance at 340nm, due to the conversion of NADH to NAD, is directly proportional to the concentration of Salicylate in the sample.

Salicylate
Hydroxylase
Salicylate + NADH + H+   -------> Catechol + NAD+

‘Ready to Use’ liquid reagents

  • Single Point Calibration - wide dynamic range
  • Specific enzyme reaction - unlike the traditional ‘Trinder Reaction’
  • Rapid - assay may be performed manually in 10 minutes
  • Adaptable - kit can be run on discrete analysers
  • Consistent interpretation of results - performs consistently well on EQAS
  • Robust, easy to use methodology

The product format is as follows:

Code
Name
IFU
K9001 Salicylate Assay

A list of all the protocols for clinical chemistry analysers is available below. To request any of theses protocols, please contact us

Clinical Chemistry Analyser Paracetamol Salicylate
Aeroset Available Available
Architect Available Available
Bayer Axon Available  
Bayer Advia 1650 Available Available
Beckman Synchron CX series Available Available
Beckman Synchron LX series Available Available
Ciba Corning 550 Express Available  
Dade Dimension Available  
Encore Available  
IL Monarch Available Available
IL 600 Available Available
IL 900 Available Available
Kone Supra Ultra Available  
Paracetamol low volume protocol Available  
Paracetamol 2 reagent protocol Available  
Olympus AU2700 / AU5400 Available Available
Olympus AU640 / 600 / 400 Available Available
Olympus AU560 Available  
Roche Cobas Bio Available  
Roche Cobas Fara Available  
Roche Cobas Mira Available  
Roche Hitachi 717 – 2 reagent Available Available
Roche Hitachi 911 – 2 reagent Available Available
Roche Hitachi 911 – 3 reagent Available  
Roche Hitachi Modular Available  
Technicon RA1000 Available  
Technicon RA2000 Available  
Technicon RA-XT Available  

 

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Immunology     Clinical Chemistry    Urease Hp Tests     Instrumentation