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Cambridge Life Sciences - Products - Instrumentation - AP22 Speedy IF
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AP22 Speedy IF

The AP22 Speedy IF processor is manufactured by DAS and CE marked with respect to the In Vitro Diagnostic Devices Directive (98/79/EC). The AP22 Speedy IF processor was launched in the UK in January 2006 as part of Cambridge Life Sciences’ innovative diagnostic solution.

The AP22 Speedy IF processor is as an open system capable of running a variety of microplates ELISA and immunofluorescence assays (IFA). The flexibility of the AP22 Speedy IF processor enables the user to run a wide range of different assays or combination of assays to accommodate the laboratory workload.


Up to eight different ELISA assays can be run at the same time, providing the assays use no more than two different wash buffers and or two different sample diluents.

For IFA, the AP22 Speedy IF processor can run up to sixteen slides of any different combination of substrates and layout. By utilising the predilution tray (92 +108 positions), samples can be run at different screening dilutions (automatically selectable from the software) and different titration protocols are easily accommodated.

Specifications

Description

Principle

Automated processor to carry out:
          ELISA tests on 96 wells micro-plates
          IFA tests on glass slides

Maintenance

Daily maintenance if performed manually by the operator

Operating system

Windows 98 - XP

Power requirements

230 or 115 VAC, 50-60 Hz, 500W

Space requirements

79 x 61 x 65 cm

Weight

77Kg

ELISA Technical Specification

Specifications

Description

Sample tubes

80 x 12 mm. 80 x 2mL screw cap tubes.
Customised racks are available for other tube sizes

Predilution tubes

92 positions

Reagent racks

8 separate reagent racks hold up to 8 different sets of calibrators, controls and reagents

Reservoirs

1 x waste tank, 1 x distilled water, 2 x separate wash buffer bottles with level sensors

Microtitre plates

2 standard 12 x 8 well plates with programmable incubation from ambient to 450C.

Plate washing

Single manifold strip washer with 2 rows of 8 needles, one set for dispensing the other for aspirating. Automatic height adjustment for different types of microplate

Reading system

8 independent photometric channels with mono and dual wavelength

Interferential filters

2 supplied as standard (450 nm, 630 nm), option for up to 4

Reading capability

From 0.000 to 3.000 OD, accuracy ±1% from 0.000 to 1.500 OD, ±2% from 1.500 to 3.000 OD

Dispensing

8 μL to 1000 μL by steps of 1 μL, precision ±1% with 100 μL


IFA Technical Specification

Specifications

Description

Sample tubes

80 75mm x 12 mm or 80 2mL screw cap tubes.
Customised racks are available for other tube sizes

Predilution tubes

92 positions + 108 positions (optional)

Reagent racks

1x removable reagent rack, positions for 12 prediluted controls, 4 conjugates (or 3 conjugates and 1 Evans Blue), 1 sample diluent and 1 needle wash solution

Reservoirs

x waste tank, 1 x distilled water, 1 x PBS wash with level sensors

Needle system

2 needles on a single arm (one dispense, one aspirate) with independent movement

Dispensing

From 8 μL to 1000 μL by steps of 1 μL, precision ±1% with 100 μL

Sample predilution

20 seconds for a 1/100 (10 μL to 1000μL)

Sample dispense

9 seconds per well (up to 1000 μL). CV < 2% @ 100μL

Reagent dispense

38 seconds for a 10 well slide @ 20 μL per well (including rinse). CV < 2% @ 100 μL

Slide rack

1 x 16 slide removable rack

Sample detection

Can detect down to 150 μL sample volume

Slide washing

Flux system utilising simultaneous use of dispense and aspirate probes

Additional options

Features

Description

Barcode reader

hand held device

Predilution tray

108 positions